ctb solution  (World Precision Instruments)

Journal: Frontiers in Neuroscience

Article Title: Modulation of colonic function in irritable bowel syndrome rats by electroacupuncture at ST25 and the neurobiological links between ST25 and the colon

doi: 10.3389/fnins.2022.930489

Figure Lengend Snippet: The positive labeling of dorsal root ganglia (DRGs) (not sectioned) under fluorescence microscopy. Representative photomicrographs showing the distribution of AF488/594-CTB-labeled sensory neurons in T6–L2 DRGs.

Article Snippet: The operation was performed under a body microscope, and 10 μl of 0.1% AF488-CTB solution (Invitrogen-Molecular Probes, Eugene, OR, USA) was slowly injected into the proximal colon (ascending colon and 1/2 transverse colon) at multiple points in the muscle layer, and the needle was left for 5 min after the injection to prevent extravasation of the tracer from affecting the experimental results.

Techniques: Labeling, Fluorescence, Microscopy

Journal: Frontiers in Neuroscience

Article Title: Modulation of colonic function in irritable bowel syndrome rats by electroacupuncture at ST25 and the neurobiological links between ST25 and the colon

doi: 10.3389/fnins.2022.930489

Figure Lengend Snippet: The neural tracing of the colon and ST25 in the dorsal root ganglia (DRGs) (sectioned). Representative and magnified photomicrographs showing the distribution of AF488/594-CTB-labeled sensory neurons in the T6–L2 DRGs. The double-labeled neurons with AF488/594-CTB presenting in yellow. Scale bars = 100 μm.

Article Snippet: The operation was performed under a body microscope, and 10 μl of 0.1% AF488-CTB solution (Invitrogen-Molecular Probes, Eugene, OR, USA) was slowly injected into the proximal colon (ascending colon and 1/2 transverse colon) at multiple points in the muscle layer, and the needle was left for 5 min after the injection to prevent extravasation of the tracer from affecting the experimental results.

Techniques: Labeling

Journal: Frontiers in Neuroscience

Article Title: Modulation of colonic function in irritable bowel syndrome rats by electroacupuncture at ST25 and the neurobiological links between ST25 and the colon

doi: 10.3389/fnins.2022.930489

Figure Lengend Snippet: The neural tracing of ST25 in the spinal cord. Representative and magnified photomicrograph showing the distribution of AF594-CTB-labeled motor neurons in the T9–T12 spinal cord. Scale bars = 100 μm.

Article Snippet: Under 2% isoflurane respiratory anesthesia, 4 μl of 0.1% AF594-CTB solution (Invitrogen-Molecular Probes, Eugene, OR, USA) was injected subcutaneously and muscularly into the right side of ST25 using a Hamilton micro-syringe (Hamilton Company, Reno, NV, United States) ( ).

Techniques: Labeling

Journal: Frontiers in Neuroscience

Article Title: Modulation of colonic function in irritable bowel syndrome rats by electroacupuncture at ST25 and the neurobiological links between ST25 and the colon

doi: 10.3389/fnins.2022.930489

Figure Lengend Snippet: The positive labeling of the sympathetic chain. (A) Positive labeling of sympathetic chain (not sectioned) under fluorescence microscopy. (B) Representative photomicrographs showing the distribution of AF594-CTB-labeled post-sympathetic neurons in the sympathetic chain (sectioned) at the thoracic segments. Scale bars = 100 μm.

Article Snippet: Under 2% isoflurane respiratory anesthesia, 4 μl of 0.1% AF594-CTB solution (Invitrogen-Molecular Probes, Eugene, OR, USA) was injected subcutaneously and muscularly into the right side of ST25 using a Hamilton micro-syringe (Hamilton Company, Reno, NV, United States) ( ).

Techniques: Labeling, Fluorescence, Microscopy